생물정보학 세미나 공지

 

 

서울대학교 생물정보연구소와 생물정보학 협동과정 공동 주최로 특별 세미나를 아래와 같이 열고자 하오니, 많은 참여 바랍니다.

 

 

일시: 2013. 6. 7.(금) 11:00

연사: 지성욱 교수(성균과대학교 삼성융합의과학원)

장소: 25동 411호

 

Title: Genome-wide decoding of microRNA-target interactions.

Abstract: The limited number of primary transcripts in the genome has promoted interest in the possibility that much of the complexity in the regulation of gene expression may be determined by RNA regulation controlled by RNA-binding proteins (RNABPs) and/or microRNAs (miRNAs). To understand such interactions in living tissues, a genome-wide means of mapping messenger ribonucleoprotein (mRNP) sites in vivo has been developed by high-throughput sequencing of RNA isolated by crosslinking immunoprecipitation (HITS-CLIP). Especially, HITS-CLIP analysis was further applied to the problem of identifying miRNA targets, for which prediction is a major challenge since miRNA activity requires base pairing through only 6-8 "seed” nucleotides. By generating crosslinking of native Argonaute (Ago) protein-RNA complexes in mouse brain, Ago HITS-CLIP produced two simultaneous datasets—Ago-miRNA and Ago-mRNA binding sites—that were combined with bioinformatic analysis to identify miRNA-target mRNA interaction sites. Not all Ago mRNA clusters correspond to known seed sequences, leading to the discovery of a new rule for miRNA-mRNA interactions, termed “pivot pairing rule”. Furthermore, sequence conservation analysis suggested that such novel non-canonical target sites named “nucleation bulge” are predictable. Therefore, we implemented a web server called “miRTCat”: a comprehensive searchable map of miRNA target sites including non-canonical nucleation bulges, not only mapped in experimentally verified Ago-miRNA-bound regions but also predicted in all 3’ untranslated regions (3’UTRs) derived from human and mouse. Ago HITS-CLIP provides a general platform to identify functional miRNA binding sites in vivo and a solution to determining precise sequences for targeting clinically relevant sites of miRNA regulation.

 

Bio:

관심연구분야

유전체학(Genomics), 생물정보학(Bioinformatics), RNA분자생물학(RNA Molecular Biology)

 

학위 및 연구과정

1995-1999 이학사, 고려대학교 생명과학부

1999-2001 이학석사, 고려대학교 생명공학원, 생화학 전공 (지도교수: 최의주)

2001-2002 연구원, 고려대학교 창의연구단 세포사멸연구센터 (센터장: 최의주)

2002-2003 연구원, 서울대학교 생물정보연구센터 (센터장: 장병탁)

2003-2009 PhD, Rockefeller University, Computational Biology and Medicine

2009-2010 Post-doc, Rockefeller University

2010-2011 Post-doc, Cold Spring Harbor Laboratory

2011-현재 조교수, 성균관대학교 삼성융합의과학원, 삼성서울병원 미래의학연구원

 

￭최근 주요 연구업적

1. Kim KK, Ham J, Chi SW. “miRTCat: a comprehensive map of human and mouse microRNA target sites including non-canonical nucleation bulges” Bioinformatics 2013, (Accepted).

2. Chi SW*, Hannon GJ, Darnell RB*. An alternative mode of miRNA target recognition. Nature Structural and Molecular Biology 2012. 19: 321-327 (*Co-corresponding Author).

 

서울대학교 생물정보연구소

생물정보학 협동과정 공동주최