일시: 2012. 11. 19.(월) 13:00

연사: 양영익 박사(J. Craig Venter Institute)

장소: 25동 411호

Title: Metagenomic data analysis.

In this talk, I will introduce metagenomic data analysis using next generation sequencing (NGS). The session will cover my two on-going researches.

The first topic is a metagenomic sequence assembler. Recently, we proposed a method for reconstructing complete protein sequences directly from NGS metagenomic data. Our framework is based on a novel Short Peptide Assembler (SPA) that assembles protein sequences from their constituent peptide fragments identified on short reads. The SPA algorithm is based on informed traversals of a de Bruijn graph, defined on an amino acid alphabet, to identify probable paths that correspond to proteins.

In the second half of the talk, I will introduce our work on oral microbiome data analysis. A large proportion of oral microbiome species remain uncultivable which leaves us with limited information of the true behavior of these species and the interactions between species. In the light of this, our primary goal was to develop a robust in vitro biofilm-model system representing the complexity of the oral microbiome. We employed deep sequencing and community analyses to further investigate these model biofilms.