생명공학공동연구원  교수님, 직원, 연구원 여러분 안녕하세요

 

생명공학공동연구원에서 4월 29일에 진행되는 세미나 정보를 알려드립니다. 

 

- Title : Hidden RNA profiles of cells in the tumor microenvironment accurately revealed by malignant cell fraction-informed deconvolution
- 연사 : 김용수 교수님(Cancer Centre Amsterdam)
- 초청자 : 황대희 교수님(서울대 생명과학부, 생물정보협동과정 주임)
- 일시 : 2024년 4월 29일 (월) 오후 16시
- 장소: 504동 105호

abstract:
Deconvolution of bulk RNA profiles can identify hidden cell fractions and functional states in the tumor microenvironment. The malignant cells, however, are commonly the most abundant cell type and impair deconvolution by profound interpatient heterogeneity. We developed a malignant cell fraction-informed RNA deconvolution method by Bayesian integration of DNA-derived malignant fraction estimates, OncoBLADE. We evaluated OncoBLADE experimentally using bulk RNA profiles with 19 CyTOF determined cell fractions from blood of 46 individuals, and in silico using 180,177 single-cell RNA profiles from 73 lung tumors. Using malignant cell fractions, OncoBLADE achieved improved accuracy in cell fraction and cell type-specific RNA profile estimation. We tested OncoBLADE on real bulk profiles of 50 adeno- and 50 squamous lung cancer samples, revealing hidden RNA profiles of malignant cells and fibroblasts that distinguished two histological subtypes. In conclusion, multimodal deconvolution utilizing bulk DNA and RNA data accurately unveils hidden RNA profiles within the tumor microenvironment.

 

첨부: CV

 

문의: 생명공학공동연구원 김서영(880-2859 / 010-3482-7382)