서울대학교 생물정보연구소 세미나를 아래와 같이 열고자 하오니, 많은 참여 바랍니다.

 

일시: 2018년 8월 28일 오후 4:30

연사: Sangsu Bae

장소: 서울대학교 220동 6층 대회의실

 

Title : Genome editing using CRISPR-Cas system

Abstract : Genome editing with engineered nucleases such as ZFNs (zinc finger nucleases), TALENs (transcription-activator-like effector nucleases), and CRISPR-Cas9/Cpf1 derived RNA-guided endonucleases is broadly used for biomedical research, biotechnology, and medicine. In addition, CRISPR base editors that enable the direct conversion of DNA bases without producing double-stranded breaks (DSBs) of DNA were developed. Unlike ZFNs and TALENs whose DNA specificities are determined by DNA-binding proteins, CRISPR nucleases use complementary base pairing to recognize target sites. Now, CRISPR nucleases are widely exploited due to the ease of use and inexpensive cost; researchers can induce gene editing at different sites by simply altering the guide RNAs. However, CRISPR nucleases cleave not only on-target sites but also off-target sites that differ by up to several nucleotides from the on-target sites, causing unwanted off-target mutations and chromosomal rearrangements. Here I present web-based programs, named CRISPR RGEN Tools (www.rgenome.net), including a novel CRISPR design tool and a genome editing assessment tool. These tools are indispensable for gene mutation in human cells, animals and plants. Furthermore, I would like to introduce versatile applications of CRISPR nucleases such as a one-step transformation of Chlamydomonas reinhardtii by the DNA-free CRISPR, a circulating tumor DNA detection and the detailed mechanism of Cas9/Cpf1 revealed by single-molecule fluorescence imaging.

 

Education and Training

2008 – 2012 Ph.D. in Physics and Astronomy (Biophysics)

Seoul National University, Seoul, Korea

(Advisor: Prof. Sungchul Hohng, Ph.D) 

2006 – 2008 M.S. in Physics and Astronomy (Biophysics)

Seoul National University, Seoul, Korea 

1998 – 2005 B.S. in Physics and Astronomy (Physics)

Seoul National University, Seoul, Korea

  

Research Experiences

2015 – Present Assistant Professor

Department of Chemistry, Hanyang University, Seoul, Korea

2012 – 2015 Post-doctoral Fellow

Department of Chemistry, Seoul National University, Seoul, Korea

(Advisor: Prof. Jin-Soo Kim, Ph.D)

 

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